Dr. Cheruzel's undergraduate research lab has recently developed a light-activated P450 hybrid enzyme with the highest reported turnover rates within the P450 family using a ruthenium metal complex. The hybrid enzyme lacks reusability, which means the lab needs a constant production in order to carry out more research. In order to improve the reusability of the hybrid enzymes we are attempting to attach it to a solid support. So far we have seen attachment of the ruthenium complex and have methods for confirming its attachment using UV-Vis and fluorescence. No protein attachment has been confirmed yet. Completion of this project attachment is confirmed future work would involve optimizing the hybrid enzyme activity and its production.

Immobilization of Light-Driven Biocatalysts

Advisor: Dr. Lionel Cheruzel

Author: Alexander Colbert