While the formation of biofilms in infectious diseases has been investigated to understand their role in persistent infections and pathogenicity, the role of biofilms in tuberculosis patients remains unknown. There is a need for technologies that enable high throughput research examining the biofilm’s role in bacterial pathogenicity. The objective of my project is addressed in three main stages: fabrication of thickness-varying PDMS microfluidic devices for biofilm culture, on-chip growth of bacterial biofilms, and morphological characterization of biofilms via epifluorescence microscopy. The images of individual micro-chambers are analyzed by open-source computational software to measure the susceptibility of biofilms to time-varying antibiotic concentration profiles. Preliminary results show that time-varying antibiotic concentration slows biofilm growth.

Mycobacteria Smegmatis Biofilms Growth

 Response to Time-Varying Antibiotic Concentration

in Microfluidic Device 

Advisor: Dr. Benjamin Hawkins

Authors: Loc Truong