Radiation and chemotherapy damages both cancerous and healthy tissue. Solid tumor cancers have an enhanced permeation and retention effect nanoparticles can exploit. Targeted nanoparticles are suited for treatment without damage. There is a great need for biocompatible and biodegradable treatments for cancer that will be specifically absorbed by cancerous tissue.
Our experiments will determine the highest amount of natural drug that can be loaded into the hydrophobic core while retaining ideal size for tumor uptake (50-200 nm diameter). We will be attaching folic acid as a targeting moiety. The hydrophobic core (PLGA) gives a wide variety of drug loading options and provides safe degradation of the polymer. A hydrophilic shell (PEG) allows for a longer circulation time before breaking down. We are using a Multi-inlet vortex mixer which was 3-D printed to create consistent particles. We have confirmed size of our particles both with and without drug loading via DLS. AFM was used to double check size distribution and morphology. HPLC testing will provide evidence of drug loading capability and entrapment efficiency.
PLGA Nanoparticles: Synthesis via MIVM and Characterization
Advisor: Dr. Folarin Erogbogbo
Authors: George Mavroudis, Amritpal Singh