Centrifugal microfluidic discs combine microfluidics and centrifugal forces to create a lab on disc system, eliminating any need for syringe and pressure pumps used with pressure driven microfluidic devices. The project objective is to use the device system to generate 10-20 micron droplets. The project objective is achieved through a numerical simulation and experimental testing of the disc. Currently, the numerical simulation yields non-uniform droplets, which are more than 100 microns in diameter. The experimental testing includes the fabrication of centrifugal and pressure driven microfluidic discs, the testing of pressure driven microfluidic devices, and centrifugal testing of the disc. The experimental and numerical simulation results will be compared and analyzed. The benefits of generating 10-20 micron sized droplets are more reactions per use, less reagents, and speed. The benefits of lab on disc are automation, efficiency, and precision.

Centrifugal Microfluidic Droplet Generation in ddPCR

Advisors: Dr. Herbert Ernst, Sundar Sethuraman, Dr. Liat Rosenfeld

Authors: Meha Gupta, Heriberto Herrera, Merjem Muran