Creation of Multi-Inlet Vortex Mixer (MIVM) to Produce Drug Loaded Nanoparticle Therapeutics for Breast Cancer Cells

Authors: Peter Tran, David Ho, Katelyn Valladao

Advisor: Dr. Folarin Erogbogbo

Nanoparticles present a promising means of drug delivery and can reduce or eliminate side effects, reduce healthcare costs, and can enhance therapeutic uptake. Issues hindering the progress of nanoparticles include inefficient drug encapsulation, inconsistent composition, and complex biological interactions in vivo. This project addresses issues of poor drug encapsulation and homogeneity via the development of a multi-inlet-vortex-mixer designed to induce desired mixing effects for the preparation of drug-loaded lipid-polymer hybrid nanoparticles (LPHNs) created for triple-negative breast cancer spheroid therapy. The project will develop a mixer and preparation method that can produce consistent LPHNs containing a PLGA/drug core, and a conjugated lecithin/PEG shell. LPHNs are assayed against 3D metastatic breast cancer cell cultures to determine cytotoxicity. This project serves as a step towards a marketable multi-inlet-vortex-mixer that can efficiently produce consistent LPHNs continuously and acts as a basis for future projects investigating methods of therapeutic drug loading in LPHNs.